Διδακτορικό

1. Augmented Expansion Microscopy.

Expansion microscopy (ExM) is a novel method that allows super-resolution imaging with conventional optical microscopes. It consists in soaking the cells with a polymer, inducing the polymerization to form a dense meshwork throughout the cell, cross-linking the fluorophores to the polymer and, after digestion of cellular protein, rehydrating of the sample. The swelling of the polymer gel led to a four fold isotropic stretching of the sample.