PhD Positions in targeted membrane fusion using a supramolecular approach, Leiden University, Netherlands (2016)

Several PhD positions are available in the research team of Dr. Alexander Kros working on membrane fusion. The project - funded by a NWO-VICI grant - is aimed todesign a biomimetic model system able to induce for the first time in-vivo membrane fusion and study its mechanism.

Fusion of lipid bilayers in cells facilitates the active transport of chemicals. Inspired by SNARE-mediated fusion, we are developing a model system able to in-vivo induce fusion. The model system consists of a complementary pair of lipidated peptides able to form a heterodimeric coiled coil motif at the membrane interface similar to natural fusion proteins. The mechanism of membrane fusion will be studied using biophysical techniques, with an emphasis on peptide-peptide and peptide-lipid interactions. Furthermore, membrane fusion between liposomes and live cells will be studied using optimized model systems. Here, it will be a major challenge to achieve efficient and targeted liposome transport directly into the cytoplasm of cells, circumventing endocytotic pathways. The ultimate goal of this project is to achieve targeted in-vivo fusion of liposomes in zebrafishcontrolled by the biomimetic model system. Future applications are foreseen in drug delivery and membrane engineering. For this exciting interdisciplinary research project we collaborate with renowned experts in peptide chemistry, supramolecular chemistry, biology and biophysics.

Requirements:
- A recent MSc degree in Chemistry, Life Sciences, or Biology (or equivalent);
- Ability to perform research independently;
- Ability to collaborate with other disciplines;
- Good communication skills;
- Good proficiency in the English language (both oral and written);
- Preferably experience in one or more of the following areas: peptide chemistry, supramolecular chemistry, cell culture, zebrafish, drug delivery;
- Excellent grades and references.

Further details:
http://www.academictransfer.com