PhD & Postdoctoral Positions, University of Freiburg, Germany (2015)

In a project funded by the DFG, we seek to continue our research on light-sheet microscopy using scanned Bessel beams in combination with two-photon fluorescence or the STED principle.

Background and Problem: The extended ring system of the Bessel beam is required for self-reconstruction, but excites fluorescence inside the object above and below the focal plane and thus reduces image contrast.

Approach 1: Using femto-second lasers, fluorescence inside the strongly scattering medium is excited by a two-photon process, leading to strong suppression of the unwanted fluorescence in the ring system [1].

Approach 2: The principle of stimulated emission depletion (STED) is applied to self-reconstructing beams. The unwanted fluorescence in the Bessel beam’s ring system is depleted by a second self-reconstructing Bessel beam with a helical phase

We are seeking a motivated candidate with a strong background in optics and microscopy. The candidate should perform advanced experiments, theoretical considerations and computer simulations to better understand complex paths of correlated and uncorrelated photons propagating through scattering media. We want to develop superior imaging methods by investigating artificial media like bead clusters, but also various fluorescent labeled biological specimen.

Further details:
http://www.researchgate.net