Precise regulation of transcription is essential to ensure that the correct genes are expressed in the correct tissue at the correct time. At the basis of gene regulation are gene-specific transcription factors (TFs), which are essential to activate or repress transcription of their target genes in specific tissues. Previous work from our and other labs (Pomp, Mol Cell, 2024) have shown that many TFs bind to DNA cooperatively, but how this cooperativity is established is largely unclear.