PhD Positions or Postdocs in Single-molecule Studies of Chromatin Replication at Delft University of Technology

In recent years, it has become possible to reconstitute the activity of eukaryotic replisome in biochemical experiments, including on chromatin substrates. Here, you will take on the exciting challenge of understanding the dynamics of chromatin replication by studying this key biological process at the single-molecule level. This includes examining the advance of the replisome in the face of nucleosomes, and the transfer and loading of nucleosomes onto the daughter strands by histone chaperones, among other topics. To do this, you will utilise our fluorescence imaging and single-molecule force spectroscopy systems. You will be involved in the purification and labelling of proteins as necessary. You will analyse resulting datasets using biophysical modelling, and discuss extensively with collaborators in the Diffley Lab (Francis Crick institute) in the areas of molecular biology and biochemistry.